Antioxidant capacity of Pinus sylvestris L.polyphenlols in bovine pulmonary artery endothelial cells

Objective To investigate protective effect of Pinus sylvestris L.polyphenlols extracts on H₂O₂ induced damage in bovine pulmonary artery endothelial cells (BPAECs).Methods Oxidative damage of BPAECs was induced by H₂O₂.The BPAECs were divided into control group,H₂O₂ model group,and high-,midium-,and low-dose polyphenlols groups (0.5,0.1,0.05 mg/mL).The influence of Pinus sylvestris L.polyphenlols extracts on H₂O₂ induced damage in BPAECs was assessed by measuring the cells' viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrozolium bromide(MTT)assay.The superoxide dismutase (SOD);malondialdehyde (MDA),and glutathione peroxidase (GSH-Px)content were determined with assay kits.Results The cell viability of BPAECs decreased (0.71±0.03%)in H₂O₂ model group compared to that of the control group.The kinases activity of SOD,catalase (CAT),and GSH-Px decreased (24.67±0.69,41.95±0.58,24.38±1.67 U/mg/prot).Meanwhile,the content of MDA increased (5.83±0.04 mmol/mL).In high-dose group (0.5mg/ml),the cell viability of BPAECs raised remarkably (1.64±0.03%)and the kinases activity of SOD,CAT,and GSH-Px improved obviously (53.62±1.24,61.01±1.10,and 48.62±0.82 U/mg/prot).The contents of MDA and protein carbonyl reduced markedly (1.96±0.00 mmol/mL,1.13±0.08 U/mg protein)compared to those of the H₂O₂ model group.The Results showed a dose-effect relationship.Conclusion The Pinus sylvestris L.polyphenlols extracts possess protective effect on H₂O₂ induced damage in EPAECs