Detection of blaNDM-1 genne and drug resistance of three Enterobacter cloacae strains isolated in Hebei province

ObjectiveTo detect New Delhi metallo-β-lactamase-1(bla_NDM-1)gene sequence and drug resistance of three Enterobacter cloacae strains isolated in Hebei province in 2012.MethodsThe isolated strains were identified by biochemical identification and bla_NDM-1 gene was detected by PCR,then sequenced and the drug susceptibility test was conducted for the strains.ResultsThe three strains were identified as Enterobacter cloacae carrying bla_NDM-1 by all the tests.The DNA sequences exhibited complete identity with the sequences that had been identified in other countries.The results of drug resistance test showed that all the three strains were sensitive to aminogly coside antibiotics and the two strains(12280010 P1 and 12280010 P2)were sensitive to fluoroquinolones antibiotics.ConclusionThe results indicate the prevalence of Enterobacter cloacae carrying bla_NDM-1 gene and with serious drug resistance,and the surveillance on the bacterium is necessary.