Interaction of tryptophan hydroxylase isoform genes in unipolar depression patients

ObjectiveTo explore the interaction between tryptophan hydroxylase-2(TPH-2)gene rs11178997 and G1463A in uniplor deression patients.MethodsWe collected peripheral blood specimens from 600 uniplor depression patients and 600 healthy controls in a mental hospital in Harbin city.PCR was used to detect the polymorphisms of rs11178997 and G1463A.The amplification of novel TPH2 gene rs11178997,G1463A was executed by TaqMan real-time-polymerase chain reaction(real-time-PCR).ResultsThe single nucleotide polymorphism(SNP)frequences of rs 1178997 and G1463A were not deviant from Hardy-Weinberg equilibrium in both case and control groups(P>0.05).Compared with the control group,significant differences in genotypes and alleles of TPH2 gene rs11178997 were observed in patient group(23.0% vs.48.7% for A/A,43.0% vs.10.0% for A/T,34.0% vs.41.3% for T/T,44.5% vs.53.7% for allele A,and 55.5% vs.46.3% for allele T;P<0.05 for all).Compared with the control group,significant differences in genotypes and alleles of TPH2 gene G1463A were observed in patient group(40.7% vs.39.7% for A/A,11.8% vs.1.3% for A/G,47.5% vs.59.9% for G/G,46.6% vs.40.3% for allele A,and 53.4% vs.59.7% for allele G;P<0.05 for all).Compared with the control group,significant differences in genotypes and alleles of TPH2 gene rs11178997,G1463A SNP were found in patient group,respectively(χ²=57.766,P=0.000;χ²=182.72,P=0.000;χ²=9.538,P=0.000;χ²=20.173,P=0.002).The interaction of TPH2 gene rs11178997 T⁺ and G1463A A⁺ was associated with depression(odds ratio=52.786,P<0.05).ConclusionThe interaction between rs11178997 and G1463A plays an important role in the pathogenesis of uniplor depression.