Effect of simazine on proliferation of MN9D

ObjectiveTo investigate the effect of simazine on proliferation of MN9D cells.MethodsThe MN9D cells in vitro were treated with simazine at concentrations of 0 μmol/L,50 μmol/L,300 μmol/L,and 600 μmol/L and with 0.5% dimethyl sulfoxide(DMSO)as solvent control.The effect of simazine on the survival rate of MN9D was detected with methyl thiazolyl tetrazolium(MTT)assay.Morphological change of MN9D cells was observed with inverted microscope.ResultsSimazine damaged the morphology of MN9D cells.Compared to that of the solvent control group,all of the survival rate of MN9D cells in the high dose group(85.62±1.07%,71.92±0.43%,67.42±0.45%,60.04±0.11%,48.71±0.76%,and 40.63±0.86% for 12,24,36,48,60,and 72 hours)decreased significantly(P<0.01 for all).With the increase simazine dose and exposure time,the survival rate of MN9D cells decreased with a dose-time dependent manner.ConclusionHerbicide simazine has inhibitive effect on proliferation of MN9D cells.