Inhibitory effect of luteolin on aminopeptidase N in glioma cells and its mechanism

ObjectiveTo study inhibitory effect of luteolin on aminopeptidase N(APN)in glioma cells and its mechanism.MethodsEnzyme inhibitory kinetics was adopted to analyze the inhibitory effect and the kinetic constant of luteolin on APN.Growth inhibition test was conducted to assess the apoptosis of U87 glioma cells.ResultsAs a competitive inhibitor of APN,luteolinn could induced apootosis in U87 glioma cells,with a half inhibition concentration(IC₅₀)of 70.85 μmol/L and an inhibition constant(Ki)of 24.83 μmol/L.Inactivation time process analysis showed that mignonette element could react with APN and reduce the activity of APN rapidly.ConclusionLuteolin is a competitive APN inhibitor and could reduce the activity of APN,playing an important role in the apoptosis of U87 glioma cells.