Inhibitive effect of juglone on adhesion and invasion of human ovarian cancer SKOV3 cells

ObjectiveTo investigate inhibitive effect of juglone on metastasis of human ovarian cancer SKOV3 cells.MethodsHuman ovarian cancer SKOV3 cells were cultured with juglone in vitro.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT)was used to investigate cell proliferation.The ability of adhesion and invasion were detected with fibronectin assay and Transwell assay.The expressions of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MM-9) proteins were determined with Western blot.ResultsJuglone could significantly inhibit the growth of SKOV3 cells in dose-dependent manner,with the cell proliferation inhibitory rates of 16.8±3.4%,39.6±9.7%,63.3±13.6%,and 84.5±17.3% at the dosages of 12.5,25.0,50.0,and 100.0 μmol/L,respectively.Compared with the control group,the adhesion rate and invasion of SKOV3 cells were 49.2 ±6.5% and 0.627±0.064,37.3±2.9% and 0.419±0.018,and 24.7±3.7% and 0.165±0.014) for 25,50,100μmol/L juglone groups(P<0.05 or P<0.01).The expressions of MMP-2 and MM-9 proteins in juglone groups were decreased with a dose-dependent trend(P<0.05 or P<0.01).ConclusionJuglone could significantly inhibit the adhesion and invasion of SKOV3 cells and the effect may be related to the inhibited expressions of MMP-2 and MMP-9 in SKOV3 cells.