Pesticide resistance of houseflies and its countermeasures in oil mining areas

Objective To explore pesticide resistance of houseflies and its countermeasures in mining areas of Shengli petroleum field.Methods Houseflies were collected with fly-trap method and pesticide resistance of their fist or second generations raised in the laboratory were tested with topical application method.Pesticide resistance-related proteins and enzyme activities in the houseflies were determined simultaneously.Results High-resistance of the houseflies to deltamethrin,beta-cypermethrin,and permethrin was observed,with the resistance coefficients(R/S)of 22.86,24.12,and 19.37,respectively,and a declined trend for the resistance to dichlorvos.After selection breeding for resistance to beta-cypermethrin,the activities of acetyl cholinesterase,catalase,and glutathione S-transferase of the houseflies were increased markedly to 9.012,10.012,and 421.35 U/mgprot.The resistance to beta-cypermethrin of the housefly populations showed a natural decline without selection pressure.Conclusion Comprehensive measures for housefly control,reasonable application of pesticides,and regular pesticide resistance monitoring should be adopted to restrain pesticide resistance of houseflies in oil mining areas.