Effects of Vaccinium vitis procyanidin on growth of glioma cells in vitro

Objective To investigate the effects and mechanism of Vaccinium vitis procyanidin on the growth of glioma cells.Methods Glioma cells were cultured and then randomly divided into a control group and three experimental groups administered with Vaccinium vitis procyanidin at the doses of 10, 20, and 40mg/L.The growth of the cells was detected with(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT) assay; the morphology of cells was observed with inverted microscope; the cell cycle was detected with flowcytometry assay; and the expression of cyclin D1 protein was detected with Western blot.Results The results of MTT assay showed that the growth of glioma cells was inhibited by different doses of Vaccinium vitis procyanidin; compared with that of the control group, the growth of glioma cells was significant inhibited in 10, 20, and 40 mg/L Vaccinium vitis procyanidin groups at 48 and 72 hours, with the A values of 0.56±0.03, 0.42±0.07, and 0.29±0.02 and 0.71±0.09, 0.56±0.02, and 0.30±0.01, respectively, and the differences were statistically significant(all P<0.05).The decrease of cell intensity with the increment of doses and obvious shrinking of the cells in Vaccinium vitis procyanidin groups were observed with inverted microscope; flowcytometry detection revealed that the number of cells in SubG1 phase was increased and that in G2/M phase decreased; Western blot results showed that expressions of cyclin D1 protein were 2.40±0.18 and 1.82±0.20 in 20 and 40 mg/L Vaccinium vitis procyanidin groups and significantly lower than that of the control group(3.06±0.11)(both P<0.05).Conclusion Vaccinium vitis procyanidin can inhibit the growth of glioma cells in vitro, the mechanism may be related to the decrease in cyclin D1 protein expression and the impact on cell cycle.