Multi-locus sequence typing of Listeria monocytogenes isolated in Hebei province

Objective To investigate the sequence type(ST) distribution of foodborne Listeria monocytogenes(Lm) isolated in Hebei province and to provide information for molecular epidemiological study of Lm.Methods The multi-locus sequence typing for Lm described in website www.pasteur.fr/mlst was adopted to detect 69 foodborne Lm stains isolated in Hebei province; Start2 and BURST were used in data analysis and UPGMA and SplitsTree were employed to establish evolutionary tree.Results For the 69 isolates, 16 sequence types were identified and the Simposon's index of diversity was 89.98%, with a newly found stain-ST705.The preponderant STs were ST8, ST87, ST2, ST1, ST3 and ST9 among the 69 isolates.Conclusion The preponderant STs of Lm isolated in Hebei province are multiple and the distribution of STs is dispersed, suggesting genetic diversity of the isolates.