Objective To explore the protective effect and possible mechanism of Lotus-seed Milk Fer mented Product (LMFP)from acute CCl4 hepatic toxicity in mice.
Methods Ascertain the effect of lotus-seed milk fermented product on LPO of normal liver homogenate and normal hepatic homogenate excitated by cysteine and ferrous sulfate in vitro.Mice were fed with distilled water, 25%, 50%, 100% LMFP and VE, intraperitoneal injected Carbon Tetrachloride(CCl4)vegetal oil admixture in the 14th day.The examines assessing the degree of the hepaticacute damage and peroxidization were executed.Pathomorphological changes were detected by Haematoxylin eosin(HE)coloration and immunohistochemistry.
Results Lactiperoxidase(LPO)level of normal liver homogenate and hepatic homogenate excitated by cysteine and FeSO4 in LMFP groups were significantly decreased compared with the control group.Compared with the model group, Hepatocyte swell, necrosis and inflammatory cells penetration in the LMFP and VE groups were relieved, Bcl-2 express enhanced.Latic dehydrogenase(LDH)activity was decreased, LPO level was observably decreased, Superoxide dismutase(SOD)activity was significantly increased in LMFP groups.(all Significance, P < 0.05).
Conclusion LMFP can protect liver from acute CCl4 hepatic toxicity by the mechanism of anti-oxide system.