Relationship between essential hypertension and two related gene polymorphism

  Objective  To investigate the relationship of GNB3 825C/Tpoly mor phism and polymorphic HindIIIrestriction site of the Ychromosome in young aged essential hypertension.

  Methods  345 patients were enrolled with essential hyper tension and 281 healthy subjects as control group.Genomic DNAwas extracted from white blood cell.Segments of GNB3 and Ychromosome were amplified from genomic DNAby polymerase chain reaction(PCR).PCRproducts were subjected to electrophoresis in agarose gels.PCRproducts were restricted with HindIIIor BseDIrespectively.The digested products were subjected to electrophoresis in agarose gels and stained with ethidium bromide.

  Results  GNB3 825C/Tallele and genot ype fr equencies did not differ significantly between essential hyper tension and control group(P>0.05).The HindIII(+)genotype was found in 55.4% of the men in hyper tension and in 67.3% of those in contr ol.The frequency of HindIII(-)genoty pe was sig nificantly higher than that in the contr ol(P=0.03).

  Conclusion  Polymorphic HindIIIr estr ict ion site of the Ychromosome maybe is associated with young essential hypertension.