Study on protective cell immune mechanism of recombinant 31/32kDa antigens of Schistosoma japanicum

  Objective   To study the effect of cell immunity in the protective immune mechanism of recombinant rSj31/32kDa antigens of Schistosoma japonicum(rSj31/32).

  Methods   BALB/c mice were immunized three times with rSj31/32. ELISA kits were used to examine the levels of IFN-C and IL-4 before and 6 weeks after immunization. By the culture of spleen cells after challenge, IFN-C and IL-4 after stimulation with SEA were quantified by ELISA kits.

  Results   The level of IFN-Cwere increased significantly a-f ter immunized with rSj31/32(P < 0.001). There was no obvious change in the level of IL-4. With spleen cells from rSj31/32 vaccinated mice, IFN-C was the most abundantly produced cytokine in response to SEA, while IL-4 secretion was the lowest(P < 0.01).

  Conclusion   Cell immunity might play an important role in the protective immunity elicited by rSj31/32 antigen.