Effects of nonylphenol on brain cytochrome P450 gene expression of F1 generation rats

  Objective   To explore the effects of nonylphenol on brain cytochrome P450 gene experession of F1 generation rats by means of microarry technique.

  Methods   The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence.Subsequently, the cDNA probes were hybridized to the Mouse 40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed.

  Results   Sixteen identified genes expressed differently, including three cytochrome P450 genes, in which two up-regulated and one down-regulated.

  Conclusion   Nonylphenol can affect the expression of some cytochrome P450 genes of rat brain.