Objective To explore the influence of cigarette smoke extract(CSE)on NKcells activity of human.
Methods To take blood specimens from 40 health persons who were non-smoking volunteer and to separate Peripheral blood monoeuclear cell(PBMC)from blood.Every person PBMC was divided into four groups: A, B, C, D.A, B were detection groups for NK cells and C, D were detection groups for LAK cells.B, D were normal controls, CSE were not added.A, Cgroup CSE were added then cultured together and observed the influence of CSE on NK cells and LAK cells activity.To compare with activity of NK amd LAK cells by methyl thiazolyl tetrazolium (MTT) assay.
Results A group, NK cells mean activity was (43.95±5.57)%. Bgroup was (45.84±5.6)%.Both no obvious difference (P > 0.05).Compared with two groups, NK cells section activity had obvious difference (P < 0.05).C group LAK cells mean activity was (20.07±1.78)%.Dgroup is (24.50±2.17)%.both had obvious difference(P < 0.05).
Conclusion CSE can influence LAK cells activity of most individuals and NK cells activity of a few individuals.Immune sur veillance function and ability of cancer cells cleared are decreased, which may be one of causes of high incidence of cancer in smokers.
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