Study on modified immuno-PCR method for detection of HFRS antibodies

  Objective   To detect HFRS antibodies by modified immuno-PCR method.

  Methods   Immuno-PCR reaction was operated in PCR tubes, which were treated with the shining of UVray, and streptavidin was used to bridge the biotinylated antibodies and biotinylated DNA, then the DN A was amplified in the same PCR tube.The PCR production was analyzed in agarosegel.

  Results   The positive rate of specific antibodies by modified immuno-PCR was 78.5%, that detected by routine immuno-PCR and indirect EL ISA method were 62.3% and 47.7% respectively.

  Conclusion   The positive rate of specific antibodies by modified immuno-PCR was higher than that of routine immuno-PCR and indirect EL ISA methods, so modified immuno-PCR might be used to detect HFRS antibodies.