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TANG Meng, DONG Ju, WANG Xiao-na, . Orthogonal experiment of single cell gel electrophoresis assay using minimal peripheral blood samples[J]. Chinese Journal of Public Health, 2004, 20(10): 1169-1170.
Citation: TANG Meng, DONG Ju, WANG Xiao-na, . Orthogonal experiment of single cell gel electrophoresis assay using minimal peripheral blood samples[J]. Chinese Journal of Public Health, 2004, 20(10): 1169-1170.
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Orthogonal experiment of single cell gel electrophoresis assay using minimal peripheral blood samples

    Abstract
  •   Objective   To optimize the experimental conditions for single cell gel electrophoresis assay(SCGE).
      Methods   Orthogonal experiment(4 factors×3 levels)were carried out: unwinding time, gel layer number, electrophor esis time, and electrophoresis voltage.
      Results   Unwinding time was the main factor, followed by the gel layer number, electrophoresis time, and electro phoresis voltage.Furthermore, number one level under four factors was optimum level.
      Conclusion   Optimized experimental conditions consisted of 20-minute unwinding time, gel of 3 layers, 20-minute electrophoresis time, and 20 volt electrophoresis voltages.
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