Screening detection of genetically modified foods by polymerase chain reaction

  Objective   To establish the screening method of detecting genetically modified food in food stuffs by PCR amplifying the camv 35 S promoter and nos terminator.

  Methods   The DNAs were extracted from genetically modified herbicide-tolerance soybean RoundUp Ready^TM Soybean(RRS)and genetically modified insect-resistance maize Bt176 Maximaizer using modified CTAB method, and then were used as templates to amplify the grain-specific endogenuous reference genes as well as camv 35 S promoter and nos terminator.

  Results   The g rain-specific endogenuous reference genes were amplified successfully when using DNAs extracted with modified CTAB method.0.5% RRS and 0.5% Bt 176 Maximaizer could be identified by PCR detecting camv 35 S promoter, and 1% RRS could be indentified by PCR detecting nos terminator.

  Conclusion   DNA extracted from genetically modified food by using modified CTAB methods can be used as template for PCR, the PCR method of detecting camv 35 S promoter and nos terminator can be used for screening genetically modified foods.