Sequence analysis on nucleopotein(N) gene of mealses virus isolated in Shenzhen

  Objective   To explore the genetic characterization and genotype of measles viruses circulating in Shenzhen during the year of 2003.

  Methods   Throat swabs were collected for virus isolation.A 450 bp fragment of the C-terminal of the nucleoprotein (N) gene was amplified by RT-PCR and subjected to sequence and phylog enetic analysis using DANSTAR software.

  Results   Only one strain was isolated from 15 throat-swabs. N genes homog enetity was 98.4% compared with other wild-type viruses of H₁ genotype.

  Conclusion   Shenzhen circulating measles viruses were belonged to the clade H₁.