Establishment of alcohol-induced liver injury model in mice by intragastric infusion

  Objective   To explore the methods forestablishing alcoholinduced liver injuy model in mice by intragastric infusion of liquid diet containing alcohol.

  Methods   Aintragastric cannulation was implanted into mouse body using surgical techniques, and the liquid diet containing alcohol was infused continuously into mouse stomach for 4 weeks, then the alteration in serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total protein(TP), albumin(ALB), triglyceride(TG)cholesterol(CHO)and histopathological changes in liver were investigated.

  Results   Serum levels of ALT, AST and TG in ethano-l infusion mice elevated significantly compared with control mice, while the serum level of ALB in ethanol-infusion mice decreased significantly.Mice infused with ethanol developed mild to severe steatosis, slight to mild inflammation and necrosis.

  Conclusion   Significant liver injury could be induced in mice infused continuously with liquid diet containing alcohol for 4 weeks, a long-term intragastric cannulation and feeding mouse model of alcohol-induced liver injury was successfully achieved.