Study on detecting Stx2 variation in escherichia coli O157:H7 with gene probe

  Objective   To understand the distribution of Stx2 variation, Stx2vha in E.coli O157:H7 isolated from some principal host animals and human in Xuzhou.

  Methods   Stx2 variation, Stx2vha was tested using Southern-blot of particularial probe.

  Results   Among the 66 E.coli O157:H7 isolates examined, 35 was positive of Stx2vha(53%), 13 was positive of Stx2(19.7%)and 17 was negative of Stx2 and Stx2vha(25.8%).One was untyped because its band was different from the others.The 35 with Stx2vha was isolated from feces of human(9, 25.7%)、sheep(13, 37.1%)、cattle(6, 17.1%)、pigs(4, 11.4%)and chickens(3, 4.5%)respectively.

  Conclusion   The isolate with Stx2vha virulence gene were the predominant type of E.coli O157:H7 in Xuzhou, and the prinicipal host was sheep.