Study and adjuvant effects of eukaryotic express plasmid of murine IL-2 and IL-3 to immunity of BCG

  Objective   Eukaryotic express plasmid of Murine IL-2(pCDNA₃IL-2)and eukaryotic express plasmid of Murine IL-3(pCDNA³IL-3)were used as adjuvants in the study.The experiments were conducted to compare the immune modulation effects and histological lesions of the two adjuvatns to mycobacterium bovis BCG vaccine according to the levels of cellular immunity, humoral immunity and histopathology in immune organs.

  Methods   The tested animals were guinea pigs.Fifty days after twice BCG vaccination samples were detected by indirect ELISA, MTT and histopathological lesions of immune organs.

  Results   pcDNA₃IL-2 and pcDNA₃IL-3(pcDNA₃IL-3 can both effectively enhance the humoral immunity and the cellular immunity of guinea pit induced by BCG(P < 0.05 or P < 0.01)and hyperplasia of histopathological lesions of immune organs.

  Conclusion   Eukaryotic Express Plasmid of Murine IL-2 and IL-3 can both promote the humoral immunity and the cellular immunity of guinea pig induced by BCG(P < 0.05 or P < 0.01), so they can be as a kind of potential adjuvants.