Investigate on nuclear magnetic resonance spectrum and fast detecting method for hypoxia stress peptide

  Objective   To investigate dactylogram information of the hypoxia stress peptide(HSP)with the Nuclear Magnetic Resonance(NMR)and to establish the fast method for detecting the HSP to provid the new measure for screening the crowd easy to sickness.

  Methods   To detect the blood from the wistar rats treated in the hypoxic environment and the mountain sickness patients with the NMR, and detect to the HSP with the reagent dispensed by ourselves, optimization the experiment condition.

  Results   Character absorption cusp was exited at the definite wave-length tn the serum from the 104 wistar rats, but only two was exited in the control group(62 cases), there was significant difference between the two groups.The cases of the chemistry migration expressed high of the mountain sickness and the control group were 40(43)and 2(43), showing significant difference between the two groips(P < 0.01).The NMRIH character chemistry migration expressed both the group of the experiment of the mountain animal and the group of clinical patients.The reaction time and wave-length were 30min and 560nm in the method of fast detecting.

  Conclusion   The HSP was exited in the body fluid of the mountain sickness patients, the chapter of the HSP was different from the other substance as known; The method of fast detecting can provid the new medilum to select the soldier for the army and to screen the easy-sickned crowd.