Schistosoma japonicum: amplication and sequence analysis on new gene encoding arginase

  Objective   To recognize and clone the novel gene named arginase of Schistosoma japonicmn(Sj).

  Methods   The arginase gene obtained in our laboratory were analyzed by bioinformatics, the gene were recognized to be incomplete.The full-length cDNA of arginase gene was obtained by 5.-end nested PCR and was made sure by sequencing in Shenyou company.Specific primers were synthesized and were used to amplify arginase gene by PCR from cercariae cDNA library.Then, the cDNA sequences were cloned into pCDNA3.1 vector and analyzed by bioinformatics.

  Results   A new cDNA sequence was obtained.The sequence contained a full-length sequence with 1 095 bp ORF, which encodeed 364 amino acid residues.The new gene was identified to be the gene encoding arginase.Its sequences were cloned into pCDNA3.1 vector and identified by restriction analysis, PCR amplication and sequencing.

  Conclusion   The full-length cDNA sequences encoding Sj arginase were firstly sequenced and cloned, which gave the basis for further study.