Protective effects of antioxidants on ultraviolet radiation-indued DNA damage

  Objective   To investigate the role of antioxidants-butylated hydroxytoluene, vitamin C and vtiamin E in UVR-induced DNA damage.

  Methods   After extracting human peripherial blood lymphocytes and treating with butylated hydroxytoluene, vitamin C and vitamin E as well as ultraviolet radiation, the damaged degree of lymphocytic DNA was ex amined by single cell gel electrophoresis.

  Results   With the UVR dose increasing, the damaged degree of lymphocytic DNA increase gradully; after treating with three antioxidants UVR-induced DNA damage was reduced, the effects butylated hydroxytoluene and vitamin C relate to its concentration and vitamin E showed dose-dependent manner.

  Conclusion   Butylated hydroxytoluene, vitamin C and vitamin E possessed antagonism to UVR-induced DNA damage but butylated hydroxytoluene and vitamin C possessed dual nature, vitamin E was a sort of perfect antioxidants in antagonism to UVR-induced DNA damage.