Change of APRPs in sera of rats with differentheat treatments

  Objective   To study the changes of several kinds of APRPs of rat with differentheat treatments.

  Methods   60 male SD rats were randomly divided into control group, heat exposure group, heat exhaustion group and preheat treating group.Using rocket immunoelectrophoresis and radial immunodiffusion, the contents of ceruloplasmin(CP), haptoglobin (HP), fibronection(Fn)in sera were determined.

  Results   the contents of CP in all groups increased sig nificantly compared with the control group, and that in preheat treat group increased significantly compared with the heat exposure group or heat exhaustion group(P < 0.05);the contents of HP in heat exposure group, the preheat treating group increased significantly compared with the control group or the heat exhaustion group(P < 0.05);the contents of Fn in heat exhaustion group decreased significantly compared with the other three groups(P < 0.05).

  Conclusion   CP, HP, Fn were relatively sensitive biomarkers of heat; the increases in CP, HP might be regarded as a sign of self-defensive response, and the decrease in Fn sugg ested that rats were damaged by acute heat.