Determination on HBV, HCV, syphilis and HIV infection in population of health checkup before marriage

  Objective   To study HBV, HCV, syphilis and HIV infections in the population of health checkup before marriage in Beijing.

  Methods   HBsAg, anti-HCV and anti-HIV were detected by enzyme-linked immunoso rbent assays (ELISAs), syphilis was tested by a rapid plasma regain assay(RPR)and a T reponema pallidum hemagg lutination assay (TPHA).Viral load of HBV and HCV were measured by fluorescence quantitative poly merase chain reactions(FQ-PCR), and HBV and HCV genotypes were determined by PCR using type-specific primers.

  Results   In 800 individuals of health checkup before marriage in Beijing, the prevalence rates of HBV, HCV infections were 12.0% and 6.0%, respectively.No syphilis and HIV infections were detected.In 96 HBsAg positive individuals, 88.5% had the HBV loads less than 10⁵ copies/ml, and 11.5% had more than 10⁵ copies/ml.18.6% were infected with HBVB genotype alone, 42.4% coinfected with B and C genotypes, and 23.7% with genotype C alone.3 HCV individuals were all infected with genoty pe1b.

  Conclusion   The HBV and HCV prevalence rates were relatively higher, and no syphilis and HIV infections were detected in the population of health checkup before marriage in Beijing.