Multiplex PCR for identification of staphylococcus aureus enterotoxin gene types

  Objective   To establish a sensitive and speedy multiple po lymerse chain reaction(multiplex PCR)technique for staphyloccus aureusentero to xin gene types.

  Methods   Template DNA of bacteria was prepared by lysostaphine and Proteinase K.130 strains staphylococcus aureus were amplified by multiplex PCR strategy.

  Results   The SEA gene positive rate of 130 staphylococcus aureus was 23.1%(30/130).The SEB, SEC, SED, SEE gene positive rates of 130 staphylococcus aureus were 43.1%(56/130), 11.6%(15/130), 6.12%(7/130)and 2.31%(3/130)respectively.The femA gene was used as a PCR internal control to avoid the presence of fals-enegative results.

  Conclusion   The multiplex polymerase chain reaction was a specific sensitive and speedy method for the detection of staphylococcus air eusenterotox in genes.