Effect of soybean isoflavone on ICF-1 expression of osteoblstic cells

  Objective   To study the effect of soybean isoflavone on IGF-1 expression of osteoblastic cells.

  Methods   The osteoblstic cells from cranial bone of new born rat was cultured.The effect of soybean isoflavone on osteoblastic cells proliferation was measured by MTT.The Streptavidin-Peroxidase Histostain TM-Plus Kits was used to detect IGF-1 expression.ICF-1mRNA was detected with situ hybridization.The quantitative analysis was monitored with HP IAS-1000 photograph analysis meter.

  Results   The IGF-1 and IGF-1 mRNA expression of the third generation osteoblastic cells cultured with soybean isoflavone was positive expression and was higher signifiantly than negative control group(P < 0.05).The plasm of positive cells was brown and yellow expression.

  Conclusion   The soybean isoflvone may affect the formation of osteoblastic cells through promoting the secretion of IGF-1.