Study on coexpression of HIV-1 gag/IFN α-2b fusion gene in vaccina virus

  Objective   pJ38 was used as expression vector, gag gene encoding type iv core protein of AIDS virus(HIV) and encoding interferon A-2b gene of human were inserted into the downstream of the hybrid promotor, where HA gene of the vaccinia virus unnecessary areas was the flank and there were 38p7.5 promotor of mutant-type vaccinia virus, A-type inclusion late promotor of vaccinia virus.AvJ38gag/IFN α-2b was acquired by homologous recombination with the natural vaccinia virus as selection of the HA-plaque for reporter.

  Methods   The IFA, Dot-blot, SDS-PAGE, Western-blot and FCM were used.

  Results   The recombinant viruses could express gag/IFN α-2b, whose molecular weight was 60 kDa.In the mice immunization experiment, the OD490 value of the serum antibodies of HIV-1 was different(P < 0.001)in groups, There was not difference between containing IFN α-2b and vJ38Gag.

  Conclusion   Gag/IFN α-2b proteins both had good reactinogenicity and immunogenictiy.