Effect of streptozocin on LPO in rat microsomal models

  Objective   To investig ate the effect of streptozocin on LPO in rat microsomal model and its dose-effect relationship.

  Methods   Hepatic microsome was extracted from clean male SD rats by calciumion precipitating.Three LPO models stimulated by CCl₄, CHP and Vc/Fe²⁺ were built respectively.Streptozocin was added into three models and regulated to final concentration: 0.3, 0.6, 0.9, 1.2, 1.5, 1.8 mg/ml.MDA, a LPO product, was deter mined after the reaction.

  Results   Compared with control model, the MDA in all trial groups was significantly increased(P < 0.01).With streptozocin increasing in trial groups, MDA was increased correspondingly, which showed a linear dose-effect relationship between streptozocin and MDA.There was a similar dose-effect relationship in three models.

  Conclusion   Streptozocin can cause oxidative damage of microsome and increase MDA product.There was a dose-effect relationship between streptozocin and MDA.