Study on differention of mouse embryonic stem cells cultured in BRL-CM to neural precursor cells in vitro

  Objective   To investigate whether buffalo ratliver cell-conditioned medium(BRL-CM)can be used as the cultur condition of embryonic stem cells(ES cells), and to get more pure neural precursor cells(NPC)of treatment.

  Methods   Mouse embryonic stem cells were cultured in BRL-CM and containing LIF medium respectively, NPC were selectively cultured in serum-free medium.Alkaline phosphatase activity was visualized with NBT/BCIP and nest in antigen was detected with immunocy tochemical methods.

  Results   BRL-CM could be used as an efficiency culture condition insted of LIF in ES cells culture.About 86% NPC were obtained from ES cells in the serum-free culture condition.

  Conclusion   BRL-CM can replace LIF and with more simple and economy; high purity NPC could be induced from ES cells with serum-free culture method.