Determination of PARP-1 gene polymorphisms with PCR-SSCP silver dying

  Objective   To investigate nucleotide polymorphisms of five exons in human poly (ADP-ribose) polymerase-1 (PARP-1) gene.

  Methods   PCR-SSCP silver staining was used to test the polymoephisms of PARP-1 gene in 634 chinese Han, Buyi and Zhuangzu people(219, 203 and 212 separatively).

  Results   Polymorphisms of 5 exons of PARP-1 gene was not detected in 219 Han people and a polymorphisms of exon20 of PARP-1 gene was detected in 203 Buyi and 212 Zhuangzu people by PCR-SSCP, Other exons were not detected.

  Conclusion   Polymorphisms may exist in exon20 of PARP-1 gene. PCR-SSCP silver staining had the advantages of simplicity, efficiency, speed and reproducibility. It has excellent potential as a tool to screen rapidly the polymorphisms PARP-1 gene and could be incorporated into molecular epidemiology study.