Effects of conjugated linoleic acid on invasion of human gastric carcinoma cell in vitro

  Objective   To investigate the effects of c9, t11-conjugated linoleic acid on cancer invasion and invision-related characteristics the metastasis cascades of tumor cells were imitated in cell culture.

  Methods   The five levels of CLA in medium were designed as 0, 25, 50, 100 and 200μmol/L. After human gastric carcinoma cell line SGC27901 were treated with c9, t11-CLA for 24 h, reconstituted basement membrane invasion assay and migration assay was used to evaluate invasive ability and migration ability of cancer cells, adhesion assay was investigated the ability of treated cells to adhere to intracellular matrix.

  Results   At the concentrations of 200, 100 and 50μmol/L, c9, t11-CLA suppressed the reconstituted basement memebrane invasion of SGC-7901 by 53.7%, 40.9% and 29.3%.Meanwhile, c9, t11-CLA could inhibit the adhesion to laminin, fibronectin and matrigel.c9, t11-CLA did not, however, significantly influence the migration ability of treated cells.

  Conclusion   c9, t11-CLA could suppress invasion of SGC-7901 cells and change some invasion2related characteristics.