Effect of methamphetamine on antioxidative enzymes and isozymes of cardiac mitochondria of mice

  Objective   To explore effects of methamphetamine (METH)on antioxidative enzymes of cardiac mitochondria of mice.

  Methods   The effect of different concentration (1.0, 1.5, 2.5 and 4.0 mg/kg) METH on the activities of glutathione peroxidase (GSH-Px) and superoxidative dismutase(SOD)of cardiac mitochondria of mice through belly injection were discussed, and the patterns of the two isozymes(GSH-Px and SOD)were attained using PAGE method.

  Results   Low concentration M ETH can activate GSH-Px and SOD while the high concentration M ETH can restrain them, and some symbol isozymes sod1, 4, 5 and gsh4 were found in the patterns of isozymes.

  Conclusion   The administration of METH on mice in vivo can lead to excessive active oxygen radicals in cardiac mitochondria which further injured mitochondrial constitute parts, including DNA and its encoding system, this maybe mainly toxicol mechanism of M ETH on mcie cardiac.