Effect of lamivudine and interferon-α on development of early mouse embryos in vitro

  Objective   To study the effects of lamivudine(3TC)and interferon-(IFN)on development of early embryos in vitro and to investigate the possibilty of antiviral therapy during first-trimester pregnancy.

  Methods   One-cell mouse embryos collected from the oviduct of the superovulated mice were cultured in microdrops of medium for 72 hours, the effects of 3TC and IFN were observed in various concentration on development of embryos, and the percentage of embryos developing to various stages of embryos was used as evaluation criteria.

  Results   The development rate of embryos was decreaed significantly in the concentration of 1 000U/ml(IFN)and 500 mol/L(3TC), two anti-HBV drugs had no inhibitive effect on development of early mice embryos under the concentrations of 500U/ml(IFN)and 300 mol/L(3TC).

  Conclusion   IFN and 3TC had no inhibitive effect on development of early embryos at the proper therapeutic concentration.