Study on immunology of recombinant vaccina virus chimeric huge protein

  Objective   pSFJ16 and pSFJ38 were used as expression vector.Both pSFJ16 and pSFJ38 contained ATI promotor and tandem repeat p7.5 mutant stage promotor.HA gene sequence of vaccinia virus was used as reporter gene.Env, gag gene encoding type ⅳ surface glycoprotein and core protein of AIDS virus(HIV)and encoding interferonA-2b gene of human were inserted into the downstream of the hybid promotor, where HA gene of the vaccinia virus unnecessary areas was the flank of vaccinia virus.This treatise was observed to humoral immunity of organism and effect of antibody induces by vJ16env/IFNα-2b and vJ38gag/IFNα-2b.Homologous recombination was occured in cell.

  Methods   Recombinat plasmids were contructed by molecule clone.The two recombinated expression plasmids and wild-type vaccinia virus were homologously recombinated by liposome transfection in Cos-7 cell with the natural vaccinia virus an selection of the HA plaque as reporter molecule.OD₄₉₀ of the serum was observed after mice were immuned.

  Results   There was significantly change between test group and negative control for response of antibody in serum.Positive control was contray(P < 0.05, P > 0.05).

  Conclusion   Recombinant vaccinia virus vJ16env/IFNα-2b and vJ38gag/IFNα-2b could stimulate micebody to produce high level antibody.The more higher effect price of protein is, the lower OD value of antibody is.