Improvement of RT-HPLC on determination of lovastatin

  Objective   To improve the detection concentration and time of plasma lovastatin by studying the RP-HPLC method.

  Methods   The condition of HPLC: The analytical column was packed with YWG-C₁₈(10μm).The mobile phase was methanol-water(83:17, V/V).The temperature of column was 25℃.The compounds were quantified using a ultraviolet detector operated at 237 nm.The lovastatin in plasma sample extracted by cycllohexane-isopropyl alcohol.

  Results   The liner range of plasma lovastatin was 2.0-80 ng/ml.The regression recation was y=241.56+197.25x(r=0.999 1).The limit of detection was 2.0μg/L and the mean recovery was(96.01±7.17)%.The determination of a sample only needed 6 min.

  Conclusion   Controlling the pre2 treatment of plasma and the ratio of methanol to water was the way to enhance the detection limit and shorten detection time.