Effect of homocystein on cell differentiation and proliferation of rat's hippocampal cells

  Objective   The effect of homocysteine on the growth hippocampal cells was investigated.

  Methods   The micro mass culture of hippocampal cell was applied to study the effect of varieties of HCY(0.01, 0.1, 1.0, 10.0, 100.0)mmol/L.

  Results   With the increased concentration of homocysteine, homocysteine inhibited the cell difference and proliferation of hippocampal cells.Promotion effect of homocysteine changed the content of protein in the cell culture which were markedly increased in image analysis and FACsS.

  Conclusion   The homocysteine obviously inhibited cell differentiation and proliferation.The teratogenic hazard of HCY may play an important role in the occurrence of neural tube defects.