Expression of multiepitope of HBV in NS-1 cell

  Objective   To study the expression of chimeric gene of the multiepitope of HBV and HBc in NS-1 cells.

  Methods   The genes encoding HBc1-72aa and 93-183aa were amplified with PCR, and multiepitope gene was synthesized. The genes were cloned into pcDNA3.1(+). The recombinant was verified by the restrict digestion and sequencing, and then transfected into NS-1 cells with lipofectin. The combined protein was detected by ELISA and IFA.

  Results   The chimeric gene, about 890 bp, was obtained by the restrict digestion and verified by sequencing. The cells transfected with the recombinant expressed the combined protein successfully.

  Conclusion   The combined protein (HBc-Mep) was expressed successfully in NS-1 cells. It laid the foundation for studying the recombinant using in DNA immunization.