Study on inhibitor of tumor angiogenesis factor

  Objective   To investigate the inhibitory effect of the low-molecular polysacchoride on tumor angiogensis factor(TAF).

  Methods   Chicken's embryonic chorio-allantois is adapted in the inhibitory experiment of TAF.In internal inhibitory experiment sarcoma line S-180 and cancer of the liver line H₂₂ and EAC line are chosen.The medicine is poured down the stomach with one time per day for 10 days consistently.To observe the inhibitory effect of the medicine on sarcoma and the life extended effect of EAC line.In external cellular-poison experiment leukocythemia line K562 and Hela cervical carcinoma line are chosen.With the method of MTT, the average cellular-growth inhibitory rate and cellular-growth inhibitory concentration of cell lines are calculated.

  Results   The low-molecular polysacceharide inhibits TAF generation of the chicken's embryonic chorio-allantois obviously.The inhibitory rates of sarcoma line S-180 and cancer of the liver line H22 are 31.7%-52.1% and 30.1%-52.1% respectively.The effects on EAC line and cancer of the liver line H22 are not distinct.IC50 of the low-molecular polysaccharide is 20μg/ml for leukocythemia K562 and is 23.4μg/ml for Hela cervical carcinoma line.

  Conclusion   The low-molecular polysaccharide is a good inhibitor of TAF.The inhibitory effects of internal inhibitory experiment and external cellular-poison experiment on tumor cell lines are all very obvious.