Anti-proliferative effect of type P53 gene mediated by adenovirus on human gastric carcinoma

  Objective   To evaluate the effect of the anti-proliferative effect of wild type P53 gene transfection mediated by recombinant adenovirus(Ad-P53)on human gastric carcinoma.

  Methods   To analyze the anti-tumoreffects of wild type P53 gene in vitro using the proliferation-defective recombinant adenovirus as the vector.The SGC-7901 cells, derived from human gastric car cinoma, were infected with the proliferatively defective recombinant adenovirus bearing wild type P53 gene(Ad-P53)or LacZgene(Ad-LacZ), the latter considered as the vector control.The effects of P53 gene on cell proliferation was evaluated by using MTT staining method or colony performing ex periment.The mechanism of the anti-proliferation was analyzed under the electro-microscopy and flow cytometry(FCM).

  Results   The transfection of wild type P53 gene into SGC-7901 cells showed anti-proliferation effects.The cell growth was inhibited by Ad-P53 infection with inhibition rate of 88.73% (MOI was 200, day 7).And the colony performing ability of the tumor cell was significantly inhibited as well, the numbers of the colonies were 22, 0 and 0, while the cells were infected with the virus of 50, 100 and 200 MOI, respectively.The SGC-7901 cells infected with Ad-P53 analyzed by FCM showed the impeding of cell grow ths and apoptosis.The percentage of apoptosis was 71.4% (MOI was 200, day 7).

  Conclusions   Wild type P53 gene was transferred into SGC-7901 cells and show ed significant anti-proliferative effects on the cell growth.The expression of the P53 gene can cause the impeding of cell growth and apoptosis.