Main respiratory viruses detected by multiplex reverse transcription polymerase chain reaction

  Objective   A multiplex reverse transcription polymerase chain reaction(mRT-PCR)method is capable of detecting and subtyping the main respiratory viruses, including influenza A(H1N1 and H3N2)and B viruses as well as respiratory syncytial virus(RSV)types A and B.mRT-PCR method is establishd in order to detect the main viruses in respiratory clinical samples and the outbreak and epidemic of influenza in Shanghai.

  Methods   Influenza A(H1N1, and H3N2), influenza B and RSV types A and B cultured by MDCK shell viral are adopted to detect their DNA zone spectrum in ultraviolet after mRT-PCR and agarose gel electrophoresis.

  Results   Corresponding five obvious DNA zone spectrums at 431 bp, 210 bp, 390 bp, 334 bp and 183 bp respectively in ultraviolet after mRT-PCR and agarose gel electrophoresis.

  Conclusions   Using five pairs of primers, mRT-PCR could be used to detect influenza virus and to subtype the influenza A and RSV in clinical samples rapidly, which is very important for diagnosis and surveillance of upper respiratory tract infectious diseases.