Application of fluorescence quantitative PCR in detection of vibrio cholerae

  Objective   To construct the detection method of vibrio cholerae with fluorescence quantitative PCR and evaluate its superiority.

  Methods   A diagnostic kit(FQ-PCR)was developed according to the common sequence of NhaA gene in vibrio cholerae.The sensitivity and speciality in detecting of vibrio cholerae in infectious water, sea food and winter-endurance specimen and clinical samples were compared with FQ-PCR kit.

  Results   In the detection of clinical samples, FQPCR and regular detection ways have the same sensitivity and speciality.While in the detection of infectious water, sea food and winter-endurance specimen, FQ-PCR has superiority to regular ways.

  Conclusion   The construction of FQ-PCR method eliminates PCR cross-contamination which cause false positive, and real-time detection ensure accurate quantity.So FQ-PCR can be used to the detection of vibrio cholerae in clinical and sanitary laboratory.