RT-PCR for rapid detection of mumps virus

  Objective   To develop the method of rapid detection of mumps virus RNA.

  Methods   1.5 pairs of primers in the nucleoprotein(NP)of the mumps virusg genome were designed.Reverse transcription polymerase chain reaction(RTPCR)and semi-nested PCR were used to amplify specific fragments of mumps virus.

  Results   357 bp and 232 bp fragments of mump virus were amplified sensitively in the clinical specimens; however, no PCR products in measles, rubella and influenza viruses.The dilution experiments showed that mumps virus could be detected as few as 10CCID ₅₀ in the first PCR and as few as 0.1CCID ₅₀ in the second PCR.By using the method, mumps virus RNA were detected directly in the clinical specimens from patient with acute mumps infection.

  Conclusion   RT-PCR was a rapid, sensitive and reliable method for the diagnosis of mumps infection.