Changes of proteins expressions of CDK2、Bcl-2 and Bax in vitamin E succinate-induced growth inhibition in Raji cells

  Objective   To investigate the effects of vitamin E succinate(VES)and vitamin E(VE)on cell growth inhibition and protein expressions of cyclin-dependent kinase-2, apoptosis-related proteins-Bcl-2 and Bax.

  Methods   Fluorescence microscope, flow-cytometry and immunocytochemical staining were used to observe the effects of VES and VE on Raji cells in vitro.

  Results   Both VES and VE could inhibit Raji cells growth.VES was more effective than VE, which inhibited cells growth and induced apoptosis in dose-dependent and time-dependent manners.Cells treated with 20μg/ml VES for 72 hours were inhibited absolutely, and the cellular growth inhibitory rate of 20 μg/ml VE was just similar to that of 5 μg/ml VES.During growth inhibition induced by VES in Raji cells, the expressions of CDK2 and Bcl-2 were down-regulated, while the expression of Bax was increased.

  Conclusion   The changes of expressions of CDK2.Bcl-2 and Bax may be related to the mechanism of anticancer biological activities of VES.