Objective To obtain stable arsenic tolerance in bone marrow mesenchymal stem cells(MSCs).
Methods Bone marrow mesenchymal stem cells were exposed atlow level arsenite.The cell surviral rate and LC50 were measured by MTT.The resistance to arsenite was observed.
Results Bone mar rowmesenchymal stem cells exposed to 1mol/L sodium arsenite for 18 weeks exhibited dramatic resistance to acute arsenite toxicity.The LC50 for acute arsenite exposure in these chronic arsenic-esposed cells was 25.8mol/L versus 11.4mol/L in control cells.
Conclusion Acquisition of stable arsenic toler ance in bone mar row mesenchmal stem cells was available.