Rapid detection of Vibrio parahaemolyticus in food by fluorescence quantitative PCR

  Objective   To constructa rapid and accurate detection method of Viborior ahaemoly ticus with fulo rescence quantitative PCR(FQ-PCR)in food.

  Methods   Adiagnodstic kitwas developed according to the gyrase gene in Vibrio Parahaemolyticus.The sensitivity and specialty of method was assessed, and the method was applied to detectartificial contaminated food samples.

  Results   The results of all eightstrains of Vibrio par ahemolyticus were positive, and the results of the other 23 strains respective belong to Vibrionaceae and other genus were negative.The quantitative detection limitof the mehtod was 10 cfu/ml in pure clutured broth, and the detection limitof the method was 10³ cfu/g and 2 cfu/g in non-cultured and o ver night-cultured ar tificial contaminated food samples respectively.

  Conclusion   FQ-PCR method has high sensitivity and specialty.The method is handy and rapid, and the whole process can finish within 36 hr.Compared with the conventional PCR method.FQ-PCR methods is insusceptible to operation contamination for the whole flow of PCR and detection shutin arreaction tube.FQ-PCR method has potential applied value in screening of food borne patho gen.