Significance of fluorescent quantitative serum HCVRNA and diagnoses in hepatitis C

  Objective   To explore the significance of serum levels of HCVRNA in hepatitis Cdiagnoses.

  Methods   The anti-HCV was monitored by indirectenzy me-linked immunosorbent assay (ELISA) and serum levels of HCVRNA quantities were tested by fluorescent quantitative PCR(FQ-PCR)in 292 cases of chronic hepatitis Cpatients.

  Results   The positive rates of anti-HCV and HCVRNA were 71.9%(210/292)and 51.3%(150/292), respectively.The rates of HCVRNA in positive anti-HCV group and negative group were 75.9%(82/108)and 10.5%(4/38), respectively.

  Conclusion   The monitored rate of HCVRNA in positive anti-HCV group patients is higher than thatof negative group.Serum levels of HCVRNA by FQ-PCR quantities are one of the bestdirectmar ks in diagnoses for gepatitis Cthatcould make up for the missed diagnoses with the mark of anti-HCV.