Objective  To investigate the awareness levels of human papillomavirus (HPV) and cervical cancer screening, as well as the acceptance of point-of-care testing (POCT) technology among women in Jinan, Shandong province, analyze the influencing factors, and assess their satisfaction with and attitudes toward the promotion of POCT technology.

Methods  A cross-sectional survey was conducted from May 2024 to June 2025 among 629 women aged 35–64 years who participated in the two-cancer screening program at two community health service centers in Jinan. Information on sociodemographic characteristics, awareness of HPV and cervical cancer screening, and acceptance of POCT technology was collected. Statistical analyses were performed with the chi-square test and binary logistic regression.

Results  A total of 629 valid questionnaires were obtained. Regarding awareness of HPV and cervical cancer screening, 46.26% of participants had heard of HPV, among whom 70.10% knew it as the primary cause of cervical cancer. Overall, 80.76% had heard of cervical cancer screening, whereas only 44.09% were aware of specific screening methods. The awareness rates of HPV testing, cytological examination, and visual inspection with acetic acid/Lugol′s iodine (VIA/VILI) were 33.86%, 24.61%, and 6.99%, respectively. Multivariable analysis showed that compared with women aged 35–44 years, those aged 55–64 years had the lowest awareness level of HPV and cervical cancer screening (OR = 0.17, 95%CI: 0.10–0.31). Compared with women with primary school education or below, those with college education or above had the highest awareness level (OR = 3.71, 95%CI: 1.44–9.56). Regarding acceptance of POCT, 55.96% of women expressed willingness to use POCT in future screening. Multivariable analysis indicated that compared with women with primary school education or below, those with senior high school education (OR = 0.43, 95%CI: 0.22–0.81) and those with college or undergraduate education and above (OR = 0.37, 95%CI: 0.17–0.81) showed increased acceptance of POCT. Freelancers (OR = 1.93, 95%CI: 1.08–3.42) and workers (OR = 2.33, 95%CI: 1.10–4.92) showed lower acceptance of POCT technology than unemployed women. In terms of satisfaction and attitudes toward promotion, 84.27% of participants believed that POCT could significantly save time and improve the efficiency of conventional screening; 83.94% believed that it could significantly improve the accuracy of conventional screening; 85.21% reported being very satisfied overall; 83.78% held a positive attitude toward its long-term application; and 83.62% were willing to recommend the technology to others.

Conclusions  Women in Jinan have established basic awareness of HPV and cervical cancer screening, while their understanding of specific screening technologies remains insufficient. Acceptance of POCT technology is at a moderate level, whereas satisfaction with and willingness to promote this technology are relatively high.