广西地区猪、鼠、狗戊型肝炎病毒感染血清学分析

Serum epidemiological survey of hepatitis E virus infection among swine, rats and dogs in Guangxi

摘要: 目的了解广西地区猪、鼠和狗血清中抗-戊型肝炎病毒(HEV)IgG抗体流行情况。方法应用HEV基因1型开放读码框(ORF)2和ORF3多肽包被酶标板,辣根过氧化物酶(HRP)标记的羊抗猪、羊抗鼠和羊抗狗建立的酶联免疫吸附试验(ELISA)法检测猪、鼠和狗血清中抗-HEV IgG。结果总抗-HEVIgG阳性率为31.84%(170/534),其中猪血清抗-HEV IgG阳性率为26.40%(66/250),鼠血清抗-HEV IgG阳性率为43.02%(77/179),狗血清抗-HEV IgG阳性率为25.71%(27/105)。结论猪、鼠和狗血清中存在HEV感染,鼠抗-HEV IgG阳性率最高。

Abstract: Objective To investigate hepatit is Evirus(HEV)infection in swine,rats and dogs in Liuzhou of Guangxi.Methods Anti-HEV IgG was detected in ser a of swine,rats and dogs by an enzyme-linked immunoabsorbent assay (ELISA),which was developed using the synthetic pept ides derived from genotype 1 HEV ORF2 and ORF3 sequences.Results T he total anti-HEV IgG positive rate was 31.84%(170/534)from all animals tested.Anti-HEV IgG positive rates of swine,rats and dogs were 26.40%(66/250),43.02%(77/179)and 25.71%(27/105)respectively.Conclusion Swine,rats and dogs could be infected by HEV,and the prevalence rate of anti-HEV IgG was the highest in rats.